Researchers from the Centre Scientifique de Monaco (CSM), the Max Planck Institute for Marine Microbiology in Bremen and the University of Kiel have succeeded in directly measuring three key parameters necessary for skeleton formation in a live tropical coral. This way, they completely characterized the carbonate chemistry at the site of calcification. The study has now been published in Science Advances.
Coral reefs are made up of massive calcium carbonate skeletons. A new study, published in Science Advances on January 16th, 2019, reveals insights into the process of calcification, namely the process that leads to the formation of these skeletons. Elucidating coral calcification is key to a deeper understanding and better predictions of how and why coral reefs respond to environmental changes, such as ocean acidification.
"By combining microscopy and microsensor measurements, we were able to directly measure calcium, carbonate and pH at the site of calcification in coral microcolonies of Stylophora pistillata and derive important carbonate chemistry parameters from it. We show that all measured and derived parameters are higher at the coral than in the surrounding seawater. This points to the importance of calcium and carbon concentrating mechanisms that are actively regulated by the coral to form its skeleton," says lead author Duygu Sevilgen, scientist at the CSM and former PhD-student at the Max-Planck-Institute for Marine Microbiology.
Microcolony of the coral Stylophora pistillata, also called Smooth Cauliflower Coral, with microsensor. (© Eric Tambutté, Centre Scientifique de Monaco)
Measuring these key parameters at the site of calcification in live coral samples (where the first crystals are formed before they fuse to build the massive skeleton) is technically highly challenging. Previous studies using microelectrodes have been carried out "blind" and were hampered by uncertainty over correct placement of the microelectrodes in the calcifying medium. As such, most of today's knowledge is based on geochemical signatures in dead skeletons, rather than measurements in live corals. Sevilgen and her colleagues overcame these difficulties by using in vivo microscopy to orientate the microelectrodes into the calcifying medium, minimizing disturbance to coral physiology. "Since many years, one of our strengths at the CSM has been to culture corals under controlled conditions and develop cutting edge techniques to analyze the physiology of living corals," says Sylvie Tambutté, research director at the CSM.
For the first time, a spe¬cial mi¬cro¬sensor de¬veloped in co¬oper¬a¬tion with the Uni¬versity of Kiel was used to meas¬ure the car¬bon¬ate con¬cen¬tra¬tion in areas just a few mi¬cro¬met¬ers in size. "Through our pre¬cise meas¬ure¬ment us¬ing spe¬cially de¬veloped mi¬cro¬sensors, we were able to make a de¬cis¬ive con¬tri¬bu¬tion to de¬term¬in¬ing the car¬bon¬ate chem¬istry at the site of skel¬eton growth. These new find¬ings lay an im¬port¬ant basis for fu¬ture re¬search con¬cern¬ing coral ad¬apt¬a¬tion mech¬an¬isms in acid mar¬ine en¬vir¬on¬ments," says Marian Hu from the In¬sti-tute of Physiology at Kiel Uni¬versity.
Alexander Venn, senior scientist at the CSM, further explains: "Lots of important data on the calcifying fluid are available in the literature on corals. However, values obtained by different methods show great variability. As a first step in our study, we combined two methods to measure pH (a pH sensitive fluorescent dye and pH microsensors) and optically verified that we measure at the same spot and at the same time. By doing this we could show that when measured at the same time and place, methods indeed agree, clarifying differences in previous findings. This highlights the usefulness and importance of optical verification during the use of microsensors to ensure proper placement within the coral."
The study presents important insights that are relevant for future microsensor and modeling approaches addressing the carbonate chemistry and dynamics during carbonate crystal formation in corals.
Story by Max Planck Institute for Marine Microbiology
Journal Reference:
Sevilgen DS, Venn AA, Hu MY, Tambutté E, de Beer D, Planas-Bielsa V, Tambutté S. Full in vivo characterization of carbonate chemistry at the site of calcification in corals. Science Advances, 2019 DOI: 10.1126/sciadv.aau7447
The work was fun¬ded by the Gov¬ern¬ment of the Prin¬cip¬al¬ity of Monaco.
